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KMID : 1007520110200041075
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Food Science and Biotechnology
2011 Volume.20 No. 4 p.1075 ~ p.1085
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Antioxidant and Anticancer Activities of Enzymatic Hydrolysates of Solitary Tunicate (Styela clava)
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Jumeri
Kim Sang-Moo
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Abstract
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The antioxidant and anticancer activities of solitary tunicate (Styela clava) hydrolysate manufactured with different proteases (Alcalase 2.4 L FG, Thermoase PC10F, and pepsin) and optimized by response surface methodology (RSM) were investigated. The hydrolysate produced by Alcalase generally had greater antioxidant and anticancer activities. Two major fractions, F1 and F2, were produced by applying Alcalase hydrolysates to Sephacryl S-100 HR gel filtration chromatography. Fraction F2 with a lower molecular weight (3.6±0.1 kDa) had higher ABTS and DPPH radical scavenging activities than F1 (5.0±1.0 kDa), with IC50 values of 11.4 and 227.5 μg/mL, respectively. Moreover, fraction F2 had higher anticancer activitiy, with IC50 values of 577.1, 1,163.3, and 887.2 μg/ mL against AGS, DLD-1, and HeLa cells, respectively. Fraction F2 was also rich in tyrosine, lysine, arginine, phenylalanine, and histidine. These results suggest that solitary tunicate hydrolysates have significant healthpromoting effects with excellent antioxidant and anticancer activities.
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KEYWORD
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anticancer, antioxidant, hydrolysate, protease, solitary tunicate
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